[An experimental study of effect of different extracts of Alisma orientalis on urinary calcium oxalate stones formation in rats]

Related Articles

[An experimental study of effect of different extracts of Alisma orientalis on urinary calcium oxalate stones formation in rats]

Zhongguo Zhong Yao Za Zhi. 2003 Nov;28(11):1072-5

Authors: Cao ZG, Liu JH, Radman AM, Wu JZ, Ying CP, Zhou SW

OBJECTIVE: To study the effect of different extracts of Alisma orientalis on urinary calcium oxalate stone formation in rats and to identify the effective constituents. METHOD: Different extracts were administered through a stomach tube to rats of different groups with renal calcium oxalate stones induced by ethylene glycol (EG) and ammonium chloride (AC). RESULT: In the rats administered with ethyl acetate elution of ethyl acetate extract, blood Cr, BUN, renal tissue calcium content, urinary calcium excretion and crystals deposition in renal tissue were significantly lower than those of the stone formation group. CONCLUSION: The ethyl acetate elution of ethyl acetate fraction extract of Alisma orientalis can significantly inhibit urinary calcium oxalate stone formation in rats and be the most effective constituent of Alisma orientalis.

PMID: 15615421 [PubMed - indexed for MEDLINE]

 Mail this post

Technorati Tags: Ammonium Chloride, Calcium Content, Calcium Oxalate Stones, Deposition, Elution, Ethyl Acetate, Ethylene Glycol, Excretion, Experimental Study, Extracts, Formation Group, Jz, Orientalis, Pubmed, Radman, Renal Tissue, Stomach Tube, Stone Formation, Zg, Zhou

[Effect of Rongshi granule on renal stone formation and osteopontin expression in rat urolithiasis model]

Related Articles

[Effect of Rongshi granule on renal stone formation and osteopontin expression in rat urolithiasis model]

Zhongguo Zhong Yao Za Zhi. 2006 Jul;31(14):1188-91

Authors: Huang P, Yang SW, Huang WH, Kong FZ, Lou YJ

OBJECTIVE: To investigate the effect of Rongshi granule on osteopontin(OPN) expression. METHOD: The urlisthiasis rats were induced by ethylene glycol (EG) and ammonium chloride, the control group rats were non-treated, and the Rongshi granule groups (low-dose group, middle-dose group and high-dose group) were administered Rongshi granule in addition to EG and ammonium chloride in 21 days. Pooled 24 h urine samples from each group were collected weekly with the use of metabolic cages, the concentration of uric calcium and oxalic acid were respectively measured by EDTA and photoelectric colorimetric method. Eight animals from each group were killed at 0, 7, 14, and 21 days, kidneys were histologic examinaed and immunohistochemical staining. RESULT: The expression of kidney osteopontin in model group was obviously higher than that of control group (P <0.01), and was up to the highest at 21 days with 1.4 times (0.281 3/0.201 of the control group. The expression of kidney osteopontin in all of the Rongshi granule groups were lower than those of model group (P < 0.05), with an obvious dose-dependent manner. The degree of the kidney calcium oxalate crystal of the rats in all the Rongshi granule groups was much lower than that of model group, and the uric calcium and oxalic acid were much lower than those of model group (P < 0.01). CONCLUSON: The Rongshi granule could inhibit the expression of osteopontin in rat urolithiasis model.

PMID: 17048592 [PubMed - indexed for MEDLINE]

 Mail this post

Technorati Tags: Ammonium Chloride, Calcium Oxalate Crystal, Colorimetric Method, Control Group, Dependent Manner, Dose Group, Edta, Ethylene Glycol, Fz, Granule, Kidneys, Model Group, Photoelectric, Pubmed, Rats, Stone Formation, Urine Samples, Wh, Yao, Yj